HL-sixty tissue were incubated which have MMRI-dos0-PE otherwise isotype manage-PE (10?g/ml) towards freeze to possess 20 min Antibody coated cells were then incubated at 37°C/5% CO2 for the indicated times to allow internalisation. After incubation, a secondary GAM IgG-AF488 antibody was applied to the samples for 20 min on ice to detect remaining surface antibody …